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3d vasculature–on–a–chip microfluidic device aim biotech 3d cell culture chips  (AIM Biotech)

 
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    AIM Biotech 3d vasculature–on–a–chip microfluidic device aim biotech 3d cell culture chips
    3d Vasculature–On–A–Chip Microfluidic Device Aim Biotech 3d Cell Culture Chips, supplied by AIM Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aim+3d+microfluidic+cell+culture+chip/3d+vasculature+on+a+chip+microfluidic+device+aim+biotech+3d+cell+culture+chips/pm39865283-159-3-7
    Average 90 stars, based on 1 article reviews
    3d vasculature–on–a–chip microfluidic device aim biotech 3d cell culture chips - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Injection:

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90.
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H2O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H 2 O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..

    Cell Culture:

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90.
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H2O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H 2 O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..

    Incubation:

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90.
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H2O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90
    Article Snippet: Tumor spheroid suspensions were pelleted via centrifugation (300 x g) and resuspended in type I rat tail collagen (Corning) at a concentration of 2.8 mg/ml (adjusted by adding 10X PBS, 0.5N NaOH, and H 2 O to reach a pH value between 7.0 and 7.5). .. The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize. ..



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    SCI-101 increases NK92-MI tumor infiltration and cell killing in a <t>3D</t> tumor spheroid model of GBM. (A) <t>Microfluidic</t> device and experimental design. GBM tumor spheroids are embedded in a collagen matrix and treated with SCI-101 as described in Materials and Methods . CFSE-labeled NK92-MI cells are then introduced to the media ports. Functional and quantitative readouts include infiltration capability of NK92-MI cells and tumor viability and growth. (B) Representative fluorescent microscopy overlaying brightfield image of NK92-MI cells (green) in U-251 tumor spheroids. NK cells infiltrate the tumor collagen chamber as indicated by a dashed arrow. Scale bar = 100 μM. The right panel quantifies the relative fluorescence of the CFSE signal in tumor spheroids, as described in Materials and Methods . *p<0.05 by the unpaired t-test. (C) Representative fluorescent microscope image of U-251 spheroids +/- SCI-101 (10 μM) and NK92-MI (E:T 5:1). (D) (left panel) Histogram quantifying normalized NK cell killing in vehicle controls and SCI-101–treated spheroids with 5 μM and 10 μM. (Right panel) NK cell killing in vehicles and SCI-101–treated (10 μM) U-87 spheroids. Values are determined by H/PI image analysis as described in Materials and Methods . *p<0.05 by the unpaired t-test. (E) Histogram quantifying the live cell area of U-251 spheroids +/- SCI-101 (5 μM) and NK92-MI (E:T 5:1). *p<0.05 by the unpaired t-test.
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    SCI-101 increases NK92-MI tumor infiltration and cell killing in a <t>3D</t> tumor spheroid model of GBM. (A) <t>Microfluidic</t> device and experimental design. GBM tumor spheroids are embedded in a collagen matrix and treated with SCI-101 as described in Materials and Methods . CFSE-labeled NK92-MI cells are then introduced to the media ports. Functional and quantitative readouts include infiltration capability of NK92-MI cells and tumor viability and growth. (B) Representative fluorescent microscopy overlaying brightfield image of NK92-MI cells (green) in U-251 tumor spheroids. NK cells infiltrate the tumor collagen chamber as indicated by a dashed arrow. Scale bar = 100 μM. The right panel quantifies the relative fluorescence of the CFSE signal in tumor spheroids, as described in Materials and Methods . *p<0.05 by the unpaired t-test. (C) Representative fluorescent microscope image of U-251 spheroids +/- SCI-101 (10 μM) and NK92-MI (E:T 5:1). (D) (left panel) Histogram quantifying normalized NK cell killing in vehicle controls and SCI-101–treated spheroids with 5 μM and 10 μM. (Right panel) NK cell killing in vehicles and SCI-101–treated (10 μM) U-87 spheroids. Values are determined by H/PI image analysis as described in Materials and Methods . *p<0.05 by the unpaired t-test. (E) Histogram quantifying the live cell area of U-251 spheroids +/- SCI-101 (5 μM) and NK92-MI (E:T 5:1). *p<0.05 by the unpaired t-test.
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    Image Search Results


    SCI-101 increases NK92-MI tumor infiltration and cell killing in a 3D tumor spheroid model of GBM. (A) Microfluidic device and experimental design. GBM tumor spheroids are embedded in a collagen matrix and treated with SCI-101 as described in Materials and Methods . CFSE-labeled NK92-MI cells are then introduced to the media ports. Functional and quantitative readouts include infiltration capability of NK92-MI cells and tumor viability and growth. (B) Representative fluorescent microscopy overlaying brightfield image of NK92-MI cells (green) in U-251 tumor spheroids. NK cells infiltrate the tumor collagen chamber as indicated by a dashed arrow. Scale bar = 100 μM. The right panel quantifies the relative fluorescence of the CFSE signal in tumor spheroids, as described in Materials and Methods . *p<0.05 by the unpaired t-test. (C) Representative fluorescent microscope image of U-251 spheroids +/- SCI-101 (10 μM) and NK92-MI (E:T 5:1). (D) (left panel) Histogram quantifying normalized NK cell killing in vehicle controls and SCI-101–treated spheroids with 5 μM and 10 μM. (Right panel) NK cell killing in vehicles and SCI-101–treated (10 μM) U-87 spheroids. Values are determined by H/PI image analysis as described in Materials and Methods . *p<0.05 by the unpaired t-test. (E) Histogram quantifying the live cell area of U-251 spheroids +/- SCI-101 (5 μM) and NK92-MI (E:T 5:1). *p<0.05 by the unpaired t-test.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Boosting Natural Killer Cell Therapies in Glioblastoma Multiforme Using Supramolecular Cationic Inhibitors of Heat Shock Protein 90

    doi: 10.3389/fmolb.2021.754443

    Figure Lengend Snippet: SCI-101 increases NK92-MI tumor infiltration and cell killing in a 3D tumor spheroid model of GBM. (A) Microfluidic device and experimental design. GBM tumor spheroids are embedded in a collagen matrix and treated with SCI-101 as described in Materials and Methods . CFSE-labeled NK92-MI cells are then introduced to the media ports. Functional and quantitative readouts include infiltration capability of NK92-MI cells and tumor viability and growth. (B) Representative fluorescent microscopy overlaying brightfield image of NK92-MI cells (green) in U-251 tumor spheroids. NK cells infiltrate the tumor collagen chamber as indicated by a dashed arrow. Scale bar = 100 μM. The right panel quantifies the relative fluorescence of the CFSE signal in tumor spheroids, as described in Materials and Methods . *p<0.05 by the unpaired t-test. (C) Representative fluorescent microscope image of U-251 spheroids +/- SCI-101 (10 μM) and NK92-MI (E:T 5:1). (D) (left panel) Histogram quantifying normalized NK cell killing in vehicle controls and SCI-101–treated spheroids with 5 μM and 10 μM. (Right panel) NK cell killing in vehicles and SCI-101–treated (10 μM) U-87 spheroids. Values are determined by H/PI image analysis as described in Materials and Methods . *p<0.05 by the unpaired t-test. (E) Histogram quantifying the live cell area of U-251 spheroids +/- SCI-101 (5 μM) and NK92-MI (E:T 5:1). *p<0.05 by the unpaired t-test.

    Article Snippet: The spheroid/collagen mixture was then injected into the center gel channel of an AIM 3D microfluidic cell culture chip (AIM Biotech) followed by 35 min incubation allowing collagen to polymerize.

    Techniques: Labeling, Functional Assay, Microscopy, Fluorescence